Composite
lacI-CEC

Part:BBa_K3254022

Designed by: Yeqing Zong   Group: iGEM19_GENAS_China   (2019-10-16)


lacI constitutive expression cassette

This part can express LacI protein constitutively. It can be used on regulating the promoters with lacO operators, such as Ptac(BBa_K864400) part and the derived Ptac promoter,such as BBa_K2572025,BBa_K3254015,BBa_K3254016 and BBa_K864407.


Usage and Biology

The functionalities of the inducible divice made up by this part and Ptac derived promoters on a P15A plasmid in E.coli DH5α host cell were described on the page of BBa_K3254025.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/sensor
Parameters
None